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ParC Aα4 Topo IV-norfloxacin-DNA ternary complexes are more cytotoxic than Topo IV-norfloxacin-DNA ternary complexes. Topo IV-, gyrase-, and ParC Aα4 Topo IV-mediated cell killing was measured in E. coli. The plating assay was performed using strain 1596 carrying pET-11c (open triangles), pET11-parC (open circles), pET11-gyrA (open squares), and pET11-parC Aα4 (closed circles).
