Figure 12.
The retinal phenotype in the adult Lama1nmf223 mouse. Fundus image (A) of the adult Lama1nmf223 mouse reveals a membrane-like structure in the vitreous (arrowhead) surrounding persistent VHP near the optic nerve head (asterisk). Fluorescein angiography (B) confirms the presence of patent vessels (arrow) within the vitreous along with the inner retinal capillaries. Anti-GFAP (red) and GS isolectin (green) labeled flatmount retina (C) shows the extension of an astrocyte membrane (arrowheads) across the retina along the persistent VHP (arrow). Asterisks indicate the optic nerve head. Cross sections (D, E) labeled with anti-PDGFRα (light blue), anti-pan laminin (red) and GS isolectin (green), and DAPI (blue) further demonstrate the astrocytes in the vitreous (solid arrowhead) above the ILM (open arrowhead) and the presence of superficial and deep capillaries in the retina (paired arrows). Diving of vitreal vessels (arrow) into the retina was also observed (E). Scale bars indicate (C: 100 μm; D: 40 μm; E: 20 μm).