Table 4.
Gene Ontology (GO) functional enrichment analysis of the flavivirus-targeted human proteins
| Ontology | Description | GO term | p-value | Associated proteins |
|---|---|---|---|---|
| Molecular function | RNA binding | GO:0003723 | **** | EIF5A, HNRPF, HNRPH3, ILF3, MATR3, MRPL20, PABPC1, PPRC1, PRKRA, RNUXA, RPS20, SSB, TAF15, TRIM21, SNRPA, XPO1, ZCCHC17 |
| Structural constituent of cytoskeleton | GO:0005200 | ** | ACTB, ACTG1, BICD1, KRT19, VIM | |
| Nuclear localization sequence binding | GO:0008139 | ** | KPNB1, NFKBIA | |
| Transcription factor binding | GO:0008134 | * | ARNTL, CAMTA2, HNRNPF, KAT5, MDF1, MED4, NFKBIA | |
| Transcription corepressor activity | GO:0003714 | * | ATN1, ENO1, RNF12, SIAH2, TSG101 | |
| Cellular component | Cytoskeleton | GO:0005856 | **** | ACTA2, ACTB, ACTG1, ACTG2, APBB1IP, AXIN1, BICD1, CASP8, CCDC99, CEP250, CEP290, CEP63, CHD3, CLIP1, DNM2, FHL2, GOPC, KIF3B, KRT19, LMNA, MLPH, MYH9, PDE4DIP, TRAF4, TYK2, VIM |
| Ribonucleoprotein complex | GO:0030529 | ** | ACTB, HNRNPF, HNRNPH3, ILF3, MRPL20, PABPC1, RPS20, SSB, SNRPA, ZCCHC17 | |
| H4/H2A histone acetyltransferase complex | GO:0043189 | ** | ACTB, KAT5 | |
| Biological process | Intracellular transport | GO:0046907 | *** | AXIN1, BICD1, DNM2, EIF5A, GGA1, GOPC, KIF3B, KPNB1, MLPH, NFKBIA, NRBP1, OPTN, RNUXA, TOM1L1, TSG101, XPO1 |
| Regulation of type I interferon-mediated signaling pathway | GO:0060338 | *** | HSP90AB1, IFNAR2, STAT2, TYK2 | |
| Regulation of innate immune response | GO:0045088 | ** | HSP90AB1, IFNAR2, NFKBIA, TRAFD1, TYK2 | |
| Viral reproductive process | GO:0022415 | ** | KPNB1, PPIA, RPS20, SMARCB1, TSG101, XPO1 | |
| Post-Golgi vesicle-mediated transport | GO:0006892 | * | DNM2, GOPC, OPTN | |
| Nuclear transport | GO:0051169 | * | AXIN1, EIF5A, KPNB1, NFKBIA, RNUXA | |
We assigned their GO features to the human proteins identified in our screen (literature plus Y2H). We then determined if these features were over-represented in comparison with the complete list of the annotated human proteins. The description of the GO enriched term (column 2), the corresponding GO identifier (column 3), the significativity of the functional enrichment test (**** p-value < = 0.0001, *** p-value < = 0.001, ** p-value < = 0.01, * p-value < 0, 05) and the associated proteins (colum 5) are given in table 4. The three GO subcategories are presented: molecular function, cellular component and biological process.