TABLE 2.
Cytokines induced by BCG-CWS in monocytesa
| Donor and stimulation | Fold induction of cytokine:
|
||||
|---|---|---|---|---|---|
| IL-1β | IP-10 | IL-12p40 | IL-6 | TNF-α | |
| A | |||||
| Control | 4.8 ± 0.8 | 15.4 ± 3.6 | 0.88 ± 0.30 | 8.7 ± 4.6 | 0.0 |
| LPS | 465.4 ± 30.1 | 47.2 ± 10.8 | 96.3 ± 26.1 | 6,413.8 ± 811.6 | 70.9 ± 10.0 |
| BCG | 4,736.6 ± 493.0 | 0.0 | 253.5 ± 100.2 | 11,790.0 ± 3,560.1 | 342.1 ± 123.1 |
| B | |||||
| Control | 8.3 ± 2.3 | 0.30 ± 0.12 | 4.6 ± 1.4 | 6.4 ± 1.0 | 0.0 |
| LPS | 546.5 ± 81.9 | 28.2 ± 7.7 | 183.8 ± 26.7 | 5,997.1 ± 323.5 | 47.9 ± 5.3 |
| BCG | 3,752.5 ± 130.3 | 0.0 | 338.5 ± 40.0 | 10,756.7 ± 1,712.8 | 280.6 ± 50.5 |
a
BCG-CWS-stimulated and nonstimulated monocytes were cultured in medium, and 24 h later, liberated cytokines were determined by ELISA. Results of two donors (A and B) are shown. IFN-β levels (not shown) were below the detection limit in both cases.