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. 2011 Nov 14;6(11):e27051. doi: 10.1371/journal.pone.0027051

Figure 2. Affinities of round 3 pools for three target proteins.

Figure 2

Selections performed against three different proteins yielded enriched aptamer pools whose binding affinities were dependent on pI as determined by a bead-based fluorescence assay. (A) PDGF-BB round 3 pool Kd = 0.10 nM. (B) Thrombin round 3 pool Kd = 0.50 nM. (C) ApoE round 3 pool Kd = 9.3 nM.