Table 1. Intraindividual variability of bile acids and C4.
Analyte | ConcMin | ConcMax | Subject A | Values <LOQ | Subject B | Values <LOQ | Subject C | Values <LOQ | Subject D | Values <LOQ |
CA | <0.020–0.049 | 0.077–4.48 | 0.599±149% | 1/24 | 0.372±155% | 0/24 | 0.039±51% | 5/24 | 0.828±154% | 0/24 |
UDCA | <0.020 | 0.048–0.138 | 0.033±89% | 10/24 | 0.009±147% | 21/24 | 0.024±69% | 11/24 | 0.037±106% | 13/24 |
CDCA | 0.039–0.163 | 0.641–2.41 | 0.489±129% | 0/24 | 0.507±105% | 0/24 | 0.322±31% | 0/24 | 0.535±120% | 0/24 |
DCA | 0.271–0.480 | 0.770–2.96 | 0.983±63% | 0/24 | 0.628±41% | 0/24 | 0.488±22% | 0/24 | 0.934±54% | 0/24 |
LCA | <0.050–0.281 | 0.102–0.799 | 0.434±29% | 0/24 | 0.350±48% | 0/24 | 0.047±51% | 14/24 | 0.234±43% | 0/24 |
GCA | 0.030–0.162 | 2.00–2.67 | 0.726±93% | 0/24 | 0.788±55% | 0/24 | 0.618±92% | 0/24 | 0.733±73% | 0/24 |
GUDCA | 0.014–0.029 | 0.186–0.311 | 0.093±83% | 0/24 | 0.077±48% | 0/24 | 0.111±73% | 0/24 | 0.088±48% | 0/24 |
GCDCA | 0.150–0.497 | 3.82–7.42 | 1.90±73% | 0/24 | 2.34±47% | 0/24 | 2.25±84% | 0/24 | 1.53±52% | 0/24 |
GDCA | 0.080–0.415 | 2.01–5.61 | 1.75±77% | 0/24 | 0.879±50% | 0/24 | 0.976±91% | 0/24 | 0.902±63% | 0/24 |
GLCA | <0.050 | <0.050–0.444 | 0.021±22% | 24/24 | 0.164±63% | 3/24 | 0.036±67% | 18/24 | 0.059±72% | 11/24 |
TCA | <0.005–0.015 | 0.136–0.700 | 0.108±167% | 0/24 | 0.059±53% | 0/24 | 0.168±95% | 0/24 | 0.043±157% | 2/24 |
TUDCA | <0.002 | 0.003–0.031 | 0.004±105% | 8/24 | 0.001±70% | 22/24 | 0.007±104% | 5/24 | 0.002±122% | 20/24 |
TCDCA | 0.019–0.033 | 0.377–2.25 | 0.356±103% | 0/24 | 0.183±58% | 0/24 | 0.563±98% | 0/24 | 0.112±89% | 0/24 |
TDCA | 0.010–0.022 | 0.161–1.88 | 0.235±190% | 0/24 | 0.076±52% | 0/24 | 0.432±107% | 0/24 | 0.079±116% | 0/24 |
TLCA | <0.005–0.005 | 0.031–0.069 | 0.021±62% | 0/24 | 0.027±50% | 0/24 | 0.015±66% | 3/24 | 0.009±76% | 7/24 |
C4 | <0.005–0.016 | 0.038–0.135 | 0.014±72% | 5/24 | 0.058±64% | 0/24 | 0.034±51% | 0/24 | 0.038±42% | 0/24 |
Primary BAs | 0.456–1.13 | 8.23–13.2 | 4.18±62% | - | 4.25±43% | - | 3.96±81% | - | 3.78±54% | - |
Secondary BAs | 0.768–1.41 | 4.00–9.32 | 3.57±56% | - | 2.21±34% | - | 2.14±70% | - | 2.34±37% | - |
Unconjugated BAs | 0.598–0.940 | 1.63–8.46 | 2.54±79% | - | 1.87±65% | - | 0.920±23% | - | 2.57±92% | - |
Conjugated BAs | 0.386–1.03 | 10.2–18.6 | 5.21±85% | - | 4.59±49% | - | 5.18±89% | - | 3.56±62% | - |
Total BAs | 1.22–2.55 | 12.2–20.7 | 7.75±59% | - | 6.46±38% | - | 6.10±77% | - | 6.13±46% | - |
Minimal and maximal concentrations and mean±variation coefficient are given for the 15 individual bile acids, C4 and the grouped bile acids for all four subjects. Concentrations are given in [µmol/L] and intraindividual variability is given as the mean concentration [µmol/L]±CV [%].