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. 2011 Nov 14;6(11):e27884. doi: 10.1371/journal.pone.0027884

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Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Dependence of FRET efficiencies on the protein expression levels of C2A to C2D domains of dysferlin. Hyperbolic fitting showed that the C2B (red), C2C (green) and C2D (purple) domains of dysferlin all mediate the self-interaction. There was no FRET for C2A construct (black). (B) Dependence of FRET efficiencies on the protein expression levels of C2E to C2G domains and the transmembrane (TM) domain of dysferlin. Hyperbolic fitting showed that the C2E (black), C2F (red), C2G (green) and TM (purple) domains of dysferlin also mediate the self-interaction. In A and B, the data were pooled for easy comparison. (C) Independence of dysferlin dimer FRET efficiencies on acceptor concentrations within the range examined. (D) Summary of FRET_max values obtained by fitting (expressed as mean ± S.E.M.). (E) Summary of K_D values obtained by fitting (expressed as mean ± S.E.M.). ND: not determined.