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. 2011 Nov 14;6(11):e27684. doi: 10.1371/journal.pone.0027684

Figure 3. DNMT1 and DNMT3a were suppressed after transfected by GLI1-siRNA in PANC-1 cells.

Figure 3

PANC-1 cells were transfected with GLI1 siRNA, 72 hours after transfection, relative expression of GLI1, DNMT1 and DNMT3a mRNA was assessed by qRT-PCR (A, B, C), while the expression of GLI1, DNMT1 and DNMT3a protein was analyzed by Western blot (D). The inset shows a substantial decrease in DNMT1 and DNMT3a expression after GLI1 interference. The results were normalized to that of β-actin expression. All data were presented as the mean ± SD of three independent experiments.