Table 1. Prevalence (%) of enterovirus RNA in fecal samples and frequency of positive samples with a high viral load according to IFIH1 genotypes.
| Enterovirus prevalence | Samples of high viral load* | ||||||
| N = 7,793 (796 children) | N = 7,309 (793 children) | ||||||
| IFIH1 SNPs† ‡ | Genotypes | Frequency % | OR | Frequency % | OR | ||
| (n children) | (n samples/n total) | (95% CI) | P-value | (n samples/n total) | (95% CI) | P-value | |
| rs35337543 | GG (788) | 12.5 (963/7,677) | 1.0 (ref) | 6.3 (483/7,676) | 1.0 (ref) | ||
| CG (8) | 8.6 (10/116) | 0.78 (0.30–2.04) | 0.61 | 5.2 (6/116) | 0.89 (0.31–2.51) | 0.82 | |
| rs35744605 | GG (786) | 12.5 (964/7,700) | 1.0 (ref) | 6.3 (483/7,699) | 1.0 (ref) | ||
| GT (10) | 9.7 (9/93) | 0.90 (0.35–2.37) | 0.84 | 6.5 (6/93) | 1.19 (0.43–3.30) | 0.74 | |
| rs35667974 | AA (752) | 12.6 (926/7,376) | 1.0 (ref) | 6.4 (468/7,375) | 1.0 (ref) | ||
| AG (44) | 11.3 (47/417) | 0.86 (0.55–1.36) | 0.53 | 5.0 (21/417) | 0.78 (0.46–1.33) | 0.36 | |
| rs35732034 | GG (788) | 12.4 (955/7,724) | 1.0 (ref) | 6.2 (477/7,723) | 1.0 (ref) | ||
| GA (8) | 26.1 (18/69) | 2.47 (0.97–6.30) | 0.06 | 17.4 (12/69) | 3.35 (1.34–8.38) | 0.01 | |
| rs1990760 | CC (135) | 10.6 (130/1,229) | 1.0 (ref) | 0.72§ | 5.5 (67/1,128) | 1.0 (ref) | 0.91§ |
| CT (386) | 13.1 (493/3,756) | 1.22 (0.91–1.63) | 6.8 (255/3,756) | 1.25 (0.89–1.74) | |||
| TT (275) | 12.5 (350/2,808) | 1.12 (0.82–1.52) | 6.0 (167/2,808) | 1.10 (0.77–1.56) | |||
SNP, single nucleotide polymorphism; OR, odds ratio; CI, confidence interval.
*Excluding enterovirus positive samples with a low-intermediate virus quantity (below 10,000 virus copies/µl enterovirus RNA).
Location of IFIH1 SNPs: rs35337543 (intron 18, 1641+1, G>C), rs35744605 (exon 10, E627X, G>T), rs35667974 (exon 14, I923V, A>G), rs35732034 (intron 14, 2807+1, G>A), rs1990760 (exon 14, A946T, T>C).
Reports of functional effects associated with IFIH1 SNPs: rs35337543 and rs35732034 influences on a putative splice site, rs35744605 is associated with loss of function (ATPase activity, dsRNA binding, truncation of protein), rs35667974 is associated with loss of function (ATPase activity, dsRNA binding), and rs1990760 is not associated with loss of function (reviewed in [29]).
Test for trend (1 d.f.).