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. 2004 Feb;72(2):774–781. doi: 10.1128/IAI.72.2.774-781.2004

FIG. 4.

FIG. 4.

Determination of nuclease activity in S. suis subcellular fractions. Proteins were analyzed on 5s DNA-containing native PA gels, and samples correspond to supernatant fractions (lanes 1 to 4) and cell wall fractions (lanes 5 to 8). Samples 1 and 5 derive from SX332, 2 and 6 derive from SX435, 3 and 7 derive from SX436, and 4 and 8 derive from SX437. Arrowheads indicate bands with nuclease activity. No activity was observed for SX435 and SX437, while the upper nuclease band was absent in SX436 samples.