FIG. 2.
Microscopic evaluation of L. amazonensis infection in MΦs. BM-MΦs from BALB/c mice (Exp. 1 and 2) or C57BL/6 mice (Exp. 3, 4, and 5) were seeded at a concentration of 1.5 × 105 cells/chamber on chamber slides. Cells were not treated or were treated with 20 ng of IFN-γ per ml for 4 h prior to infection with 3 × 105 amastigotes (Ama.) or 7.5 × 105 stationary-phase promastigotes (Pro.). After 48 h of incubation, cells were processed for immunostaining of the parasites. (A) Summary of the average numbers of parasites per cell, as calculated by dividing the total number of parasites by the total number of MΦs examined. (B and C) Representative images of amastigote-infected control MΦs (B) and IFN-γ-treated MΦs (C). A superscript a indicates that the P value is <0.01.