FIG. 4.
Enhanced amastigote replication in IFN-γ-treated MΦs. C57BL/6 BM-MΦs (3 × 105 cells/well) were not treated or were treated with 20 ng of IFN-γ per ml for 4 h before or after infection with 4.5 × 105 amastigotes. The parasite binding to the MΦ monolayer was synchronized as described in Materials and Methods. The number of intracellular amastigotes was determined at 1 h (A) and 48 h (B) postinfection. Data are the mean +/− SD for a given condition in triplicate. The results for three independent experiments are shown.