TABLE 1.
Binding of rSP-D to different strains of S. pneumoniaea
| Strain | Serotype | MLST | Binding at rSP-D concn (μg/ml) of:
|
||
|---|---|---|---|---|---|
| 0 | 5 | 10 | |||
| M393 | 3 | 260 | 0.18 ± 0.01 | 0.30 ± 0.02 | 0.34 ± 0.01 |
| M110 | 3 | 180 | 0.19 ± 0.01 | 0.27 ± 0.01 | 0.37 ± 0.04 |
| M290 | 3 | 49 | 0.13 ± 0.01 | 0.22 ± 0.02 | 0.25 ± 0.01 |
| M9 | 18C | 102 | 0.20 ± 0.02 | 0.40 ± 0.02 | 0.47 ± 0.02 |
| M241 | 18C | 118 | 0.21 ± 0.01 | 0.34 ± 0.03 | 0.46 ± 0.05 |
| M208 | 18C | 119 | 0.21 ± 0.02 | 0.31 ± 0.02 | 0.38 ± 0.07 |
| GM70 | 19A | 81 | 0.13 ± 0.09 | 0.22 ± 0.02 | 0.26 ± 0.01 |
| M41 | 23F | 81 | 0.17 ± 0.01 | 0.29 ± 0.06 | 0.32 ± 0.01 |
| M322 | 14A | 156 | 0.12 ± 0.08 | 0.19 ± 0.01 | 0.25 ± 0.01 |
| M15 | 9V | 156 | 0.10 ± 0.04 | 0.17 ± 0.01 | 0.25 ± 0.007 |
a
Binding was assessed by bacterial solid-phase ELISA. Experiments were done in 5 mM calcium. The values are means ± standard errors of the mean of optical densities at 450 nm from three or four replicate experiments. In all cases, the optical density was significantly increased compared with the control value (in the absence of rSP-D); P < 0.05.