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. 2004 Feb;72(2):871–879. doi: 10.1128/IAI.72.2.871-879.2004

FIG. 5.

FIG. 5.

Effect of general caspase inhibitor on CDT-induced apoptosis. (A) Jurkat cells were preincubated with z-VAD-fmk (100 μM) for 30 min and then were treated with CDT (1, 10, or 100 ng/ml) for 16 h. Cells were stained with FITC-annexin V and PI and analyzed by flow cytometry. The flow cytometry pattern represents CDT (100 ng/ml)-treated lymphocytes with (+) or without (−) z-VAD-fmk. Inhibition of apoptosis was calculated as the relative percentage of living cells, or the population in the LL quadrant (annexin V− PI− population). (B) z-VAD-fmk inhibits apoptosis in the cells treated with various concentrations of CDT. (C) Effect of z-VAD-fmk on caspase-3, -7, and -8, caspase-8 and -6, and caspase-9 activity induced by CDTB. Jurkat cells were preincubated with z-VAD-fmk (100 μM) for 30 min and then were treated with CDT (100 ng/ml). Caspase activity was measured as described in Materials and Methods.