FIG. 7.

Dose-dependent inhibitory effects of caspase inhibitors on CDT-induced apoptosis. Jurkat cells were preincubated with various concentrations of caspase inhibitors (25, 50, 100, and 200 μM) for 30 min and then were treated with CDT (100 ng/ml). The inhibitors used were WEHD (caspase-1 inhibitor), VDVAD (caspase-2 inhibitor), and DEVD (caspase-3, -7, and -8 inhibitor). After 16 h, cells were stained with FITC-annexin V and PI and analyzed by flow cytometry. Panels show the relative inhibition of CDT-induced apoptosis with VDVAD (A), DEVD (B), and VDVAD and DEVD (C). Inhibition of apoptosis was defined as the relative percentage of living cells (annexin V⁻ PI⁻). VAD (general caspase inhibitor) and WEHD were used at concentrations of 100 and 200 μM, respectively.