FIG. 8.

Elevation of caspase-2 or -7 activity and cytochrome c release by CDT treatment. (A) Jurkat cells were preincubated with the indicated inhibitor (100 μM) for 30 min and then were treated with CDT (100 ng/ml) for 16 h. The inhibitors used were VAD (general caspase inhibitor), VDVAD (caspase-2 inhibitor), DMQD (caspase-3 inhibitor), DQTD (caspase-3 and -7 inhibitor), and DEVD (caspase-3, -7, and -8 inhibitor). Caspase activity was measured as described in Materials and Methods. (B) Cytosol and mitochondrial fractions were extracted from Jurkat cells that were treated with CDT (100 ng/ml) for 0, 4, 8, and 16 h and were subsequently immunoblotted with an anti-cytochrome c Ab followed by a horseradish peroxidase-conjugated secondary Ab. The bands of cytochrome c were visualized by ECL.