Figure 4.
PGI2 analogs increased the ratio of IL-23/IL-12 produced by BMDCs from WT and IP−/− mice. BMDC cells were cultured in GM-CSF for 8 days. The cell population was treated with PGI2 analogs (iloprost and cicaprost) at increasing concentrations in the presence of LPS (1 μg/ml) for 24 h. The levels of IL-23 and IL-12 in the culture supernatants were measured by ELISA. Data represent the ratio of IL- 23/IL-12 at each concentration of the PGI2 analogs. *, p < 0.05 compared with vehicle- treated cells.