TABLE 3.
Signal sequence coding region of the sabA gene and deduced amino acid sequences of 18 different Helicobacter pylori strainsa
| Strain | Sequence of the signal peptide coding region | No. of CT repeats | Deduced amino acid sequence | Gene status |
|---|---|---|---|---|
| G1 | ATGAAAAAACGATTTTTACTTTCTCTATCCC--------------TTGCATCGTCATTACTTTATGCTGAAGACAACGGCTTTTTTGTGA | 3 | MKKRFLLSLSL----ASSLLYAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| M30 | ATGAAAAAACGATTTTTACTTTCTCTATCCC--------------TTGCAGCGTCATTACTTTATGCTGAAGACAACGGCTTTTTTGTGA | 3 | MKKRFLLSLSL----AASLLYAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| M32 | ATGAAAAAGACAATTCTGCTCTCTCTC--------------------GCTTCATCGCTCTTGCACGCTGAAGACAACGGCTTTTTTGTGA | 4 | MKKTILLSL------ASSLLHAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| M45 | ATGAAAAAACGATTTTTACTCTCTCTCTC--------------GCTTGCGGTATCATCGCTCCATGCTGAAGACAACGGCTTTTTTGTGG | 5 | MKKRFLLSLSL----AVSSLHAEDNGFFVGVGYQIGEAVQMVKNTGEL | On |
| G15 | ATGAAAAAACGATTTTTACTCTCTCTCTCTC------------GTTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTATCG | 6 | MKKRFLLSLS----RLRYHRSTLKTTAFLSAWAIKSAKRCKWSKTPVN | Off |
| M59 | ATGAAAAAGACAATTCTACTCTCTCTCTCTC----------------GCTTCATCGCTCTTGCACGCTGAAGACAACGGCTTTTTTGTGG | 6 | MKKTILLSLS----RFIALAR* | Off |
| G5 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTC----------GCTTGCGGTACCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGG | 7 | MKKRFLLSLSL-ACGTIAPR* | Off |
| M61 | ATGAAAAAGACAATTTTACTCTCTCTCTCTCTC--------------GCTTCATCGCTCTTGCATGCTGAAGACAACGGCTTTTTTGTAG | 7 | MKKTILLSLSL----ASSLLHAEDNGFFVGVGYQIGEAVQMVKNTGEL | On |
| G21 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTC--------GTTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGA | 8 | MKKRFLLSLSLSF--AVSSLHAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| M23 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTC--------GCTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGA | 8 | MKKRFLLSLSLSL--AVSSLHAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| G16 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTC------GCTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTATCG | 9 | MKKRFLLSLSLS--RLRYHRSTLKTTAFLSVWAIKSAKRCKWSKTPVN | Off |
| M25 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTC------GTTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTATCG | 9 | MKKRFLLSLSLS--RLRYHRSTLKTTAFLSVWAIKSAKRCKWSKTPVN | Off |
| G13 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTCTC----GCTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGG | 10 | MKKRFLLSLSLSLACGIIAPR* | Off |
| M31 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTCTC----GCTTGCGGCATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGA | 10 | MKKRFLLSLSLSLACGIIAPR* | Off |
| G26 | ATGAAAAAACGAATTTTACTCTCTCTCTCTCTCTCTCTCTC--GCTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTAA | 11 | MKKRILLSLSLSLSLAVSSLHAEDNGFFVXVGYQIGEAVQMVKNTGEL | On |
| M65 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTCTCTC--GCTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGA | 11 | MKKRFLLSLSLSLSLAVSSLHAEDNGFFVSAGYQIGEAVQMVKNTGEL | On |
| G6 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTCTCTCTCGCTTGCGGCGCCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTGG | 12 | MKKRFLLSLSLSLSRLRRHRSTLKTTAFLWARAIKSVKRCKWSKTPAN | Off |
| M38 | ATGAAAAAACGATTTTTACTCTCTCTCTCTCTCTCTCTCTCTCGTTTGCGGTATCATCGCTCCACGCTGAAGACAACGGCTTTTTTGTAG | 12 | MKKRFLLSLSLSLSRLRYHRSTLKTTAFL* | Off |
G, gastritis strain; M, MALT strain. The stop codon is boxed for strains having the off status (except for strains G15, G16, M25, and G6), and the end of the protein is indicated by an asterisk. Concerning these strains, the stop codon is at positions 133 and 144 for the G15 and G6 strains, respectively, and position 139 for the G16 and M25 strains. All 86 strains tested in this study having the on status presented the italicized conserved motif. CT repeats are underlined, and the thymidine-thymidine sequences in the middles of the repetitions are in boldface. The sabA gene sequences from G1 to M38 strains were submitted to GenBank and assigned accession no. AY299975 to AY29992, respectively.