(A) Gel electrophoretogram shows RT-PCR products of CYP27B1, IGF-I, and GAPDH in hMSCs after 0, 2, and 6 hours with 100 nM PTH1-34 in standard growth medium (10% FBS-HI).
(B) Gel electrophoretogram shows RT-PCR products of IGF-I and GAPDH in hMSCs after 6 hours with 0, 0.1, 1, 10, or 100 nM PTH1-34 in standard growth medium (10% FBS-HI).
(C) Western immunoblot shows p-IGF-IR, IGF-IR, p-CREB, CREB, CYP27B1, and β-actin in hMSCs after 0, 10, 30, 60, and 120 minutes with 100 ng/ml rhIGF-I in standard growth medium (10% FBS-HI). Results are representative of 3 independent experiments.