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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Aging Cell. 2011 Oct 7;10(6):1038–1046. doi: 10.1111/j.1474-9726.2011.00746.x

Fig. 2.

Fig. 2

Smurf2 regulates the steady-state protein levels of Id1 and Id3. The expression of Smurf2 was down-regulated by shRNA in (A) senescent fibroblasts or (D) tumor cells. Expression of Id1 and Id3 was analyzed in Western blot and quantitative RT-PCR. Relative transcript levels of Id1/Id3 in cells expressing a non-silencing shRNA (NS shRNA) were set to be 1 after normalized with β-actin. Smurf2 (S), C716A (C), C2 or GFP (G) was ectopically expressed in (B) early passage fibroblasts or (C) tumor cells. Expression of Id1 and Id3 was analyzed in Western blot and quantitative RT-PCR. Relative transcript levels of Id1/Id3 in cells expressing C2 or GFP were set to be 1 after normalization with β-actin. Error bars were calculated from standard deviations of at least three independent experiments. Two-tailed and unpaired Student t–test was used in statistical analysis, and statistic significance is indicated as: * (P<0.05), ** (P<0.01), and *** (P<0.001).