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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Aging Cell. 2011 Oct 7;10(6):1038–1046. doi: 10.1111/j.1474-9726.2011.00746.x

Fig. 3.

Fig. 3

Smurf2 interacts with Id1/Id3. (A) 293T cells were transfected with constructs as indicated. Immunoprecipitation (IP) with anti-Flag antibody was followed by immunoblot (IB) with anti-Id antibody. (B) MDA-MB-231 cells were treated with MG-132 (20 µM) for 4 hrs before cell lysis. Immunoprecipitation with anti-Smurf2 antibody or IgG control was followed by Western blot. (C) and (D) Determination of domain(s) in Smurf2 and Id1 that mediates Smurf2-Id1 interaction. Deletion constructs of Flag-tagged Smurf2 and Myc-tagged Id1 were shown schematically in (C) and (D). 293T cells were transfected with constructs as indicated. Cell lysates were subjected to immunoprecipitation with anti-Flag antibody. Precipitated immunocomplex were analyzed in Western blot with anti-Myc antibody. TCL: total cell lysates.