Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Aging Cell. 2011 Oct 7;10(6):1038–1046. doi: 10.1111/j.1474-9726.2011.00746.x

Fig. 6.

Fig. 6

Id1 mediates the regulation of p16 expression by Smurf2. (A) Smurf2 (S), C716A (C) and Id1 were expressed in TERT-immortalized human fibroblasts as indicated. Expression of p16 was analyzed in Western blot. (B) Expression of Id1 was knockdown by stably expressed shRNA in late passage human fibroblasts. In cells with Id1 down-regulation or control cells expressing non-silencing shRNA (−), shRNA targeting Smurf2 was expressed using lentiviral infection. Expression of p16 was analyzed in Western blot. p16 proteins were quantitated using NIH ImageJ, and normalized with α-tubulin. Relative p16 protein levels in non-silencing controls were set to be 1.