Figure 1.
Urea-glycerol gels showing myosin RLC bands in: (a) tarantula demembranated muscle homogenate in relaxing and activating conditions (bands used for the LC-MS/MS analysis, Fig. 3–5); (b) quick-frozen relaxed live tarantula muscle (left: homogenate, right: supernatant; see Materials and Methods). Arrows point to non-phosphorylated bands (Non-P: A, A′), mono-phosphorylated bands (Mono-P: B, B′) and to bi-phosphorylated band (Bi-P: C′); ’ indicates the activated state.