Fig. 1.

Effects of inhibitors on DHA-induced development of mouse hippocampal neurons. Hippocampal cultures were prepared from fetuses obtained on embryonic day 18 (E18) from timed pregnant C57/BL6 mice and maintained in Neurobasal medium with 2 % B27 supplement as previously described [3,22]. The cultures then were treated with DHA (1 μM) for 7 days in the presence or absence of the fatty acid amide hydrolase inhibitor URB597 (FAAHI, 1 μM), cyclooxygenase inhibitor indomethacin (50 μM) or lipoxygenase inhibitor NDGA (10 μM). Representative photomicrographs are shown at 60× magnification. Corresponding control cultures were included. After incubation, neurons were stained with MAP2 (green, a neuron-marker protein), DAPI (blue, nuclear-marker) and synapsin 1 (red). These photomicrographs are taken from reference 22.