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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2011 Jul 23;96(1-4):114–120. doi: 10.1016/j.prostaglandins.2011.07.002

Fig. 4.

Fig. 4

Effects of synaptamide (0.1 μM) on neurite growth, synaptogenesis and synaptic activity. Representative photomicrographs are shown at 60× magnification. Synaptogenesis was evaluated by the number of synapsin puncta / 10μm neurite. **, p<0.01; ***, p<0.001. For synaptic activity, spontaneous postsynaptic currents (sPSCs) including glutamatergic (Glu-sPSCs) and GABAergic (GABA-sPSCs) components were recorded in hippocampal neurons cultured with or without 0.1 μM synaptamide for 10 days. To isolate the GABAergic sPSCs, 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium (NBQX, 5 μM) and D-2-amino-5-phosphonopentanoic acid (AP5, 50 μM) were used. For measurement of glutamatergic sPSCs, bicuculline (20μM) was superfused onto the neuron. Paired t-tests were performed against the baseline value of each group (##, p<0.01; ###, p<0.001) or between indicated groups (***, p<0.001). The bar graphs are taken from reference 22.