Differential gene expression in P. gingivalis. Total RNA was isolated from P. gingivalis 33277 grown on TSB plates with (lanes 2, 4, 6, and 8) or without S. cristatus fraction AS6 (lanes 1, 3, 5, and 7) and subjected to differential display analysis. Arbitrary primer (H-AP) combinations were used in reverse transcription reactions. The fluorescent differential display gel shows the band pattern obtained from duplicate reactions. The arrows indicate the bands corresponding to cDNA fragments that were differentially expressed in the presence of S. cristatus CC5A proteins and that were cloned and sequenced.