TABLE 2.
Synthetic oligonucleotide primers used for PCR and RT-PCR
| Primer | Sequence (5′ → 3′) | PCR product |
|---|---|---|
| pg2167for | TAGCCCACAATAAGGCTTTG | 224-bp fragment of pg2167 gene |
| pg2167rev | CGTGCATCATCATCGGCATA | |
| pg2131for | AGCTTACCAGGTAGGGAAAT | 143-bp fragment of pg2131 gene |
| pg2131rev | GAAGCATAGCCTACCATGGA | |
| pg1361for | CTTCATTCCGCACACTCGAT | 362-bp fragment of pg1361 gene |
| pg1361rev | CACCATACTCTGATCCATTC | |
| pg0707for | TTGGGCTTGAAAGTGGGTTA | 162-bp fragment of pg0707 gene |
| pg0707rev | ATGTAGCCTGTACGCGGCTG | |
| pg2167for2 | CTCTTCGGAATGTCTACG | 1.4-kb fragment of pg2167 gene |
| pg2167rev2 | TACAACTAACTCTTATCG | |
| RGP1 | TCAACACCGGTAGAGGAAAA | 900-bp fragment of rgp gene |
| RGP2 | AATGGTGCTGGCGATAATAG | |
| FE1 | CGGGATCCCGTGGTATTGAAGACCAGCAAT | 1,042-bp fragment of fimA gene |
| FE2 | GGAATTCCAAGTAGCATTCTGACCAACGAG |