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. 2011 Nov 2;8:149. doi: 10.1186/1742-2094-8-149

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Copyright ©2011 Virgili et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence staining for CD3 and CD20 in spinal cord slices from vehicle-administered (A and B, respectively) and diazoxide-treated EAE mice (C and D, respectively). Bottom panel shows higher magnification of CD3-positive cells (C). Upon quantification, no significant differences between vehicle- and diazoxide-treated mice were observed for the numbers of CD3- and CD20-positive cells in any of the spinal cord regions analyzed (E and F, respectively). Results are expressed as mean ± SEM. n (animals) per group ≥ 6. Slices analyzed per animal and section ≥ 3. Scale bar = 100 μm.