Figure 5. Peritransplant anti-LFA-1 mAb inhibits donor-reactive CD8⁺ and CD4⁺ T cell development to IFN-γ producing cells.

C57BL/6 mice were treated with or without 200 μg anti-LFA-1 mAb on days −1 and 0 and then received complete MHC mismatched A/J cardiac allografts on day 0. On the indicated day post-transplant, aliquots of unseparated spleen cells, enriched CD4⁺ or enriched CD8⁺ T cells from the recipients were cultured overnight with syngeneic or donor stimulator cells on anti-IFN-γ mAb coated filters. Numbers of donor-reactive T cells producing IFN-γ were determined by ELISPOT. Numbers of spots in co-cultures with syngeneic stimulators were always < 3 spots/5 × 10⁵ stimulator cells. The data shown are representative of two independent experiments.