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. 2004 Feb 17;90(4):892–899. doi: 10.1038/sj.bjc.6601589

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Copyright © 2004 Cancer Research UK

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T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with AmpliTaq Gold polymerase and AmpliTaq Gold buffer (A), Promega Taq and Promega buffer (B), Promega Taq and AmpliTaq Gold buffer (C) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer (A, C).