IFN-γ (A) and IL-10 (B) production in PBMC from Buruli disease patients with nodular and ulcerative forms of the disease. Cytokine levels in 7-day culture supernatants of PBMC stimulated with M. bovis BCG, M. ulcerans, and Ag85 from M. bovis BCG were measured by ELISA as described in the Materials and Methods section. Data are represented as individual values obtained for five subjects with nodules (group 1) and nine with ulcers (group 2). *, P < 0.05, Wilcoxon sum rank test, compared to production in patients with nodules.