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. 2011 Oct 28;9:74. doi: 10.1186/1741-7007-9-74

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Copyright ©2011 Elmore et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Small ubiquitin-like modifier (SUMO) is required for the localization of Ulp1^(C580S)to the septin ring. (A) The indicated mutants smt3-331, ubc9-1, smt3-R11, 15, 19, siz1Δ siz2Δ (YOK 1995, YOK 2065, YOK 1910 and YOK 2067) and a WT control strain (WT) were transformed with a plasmid expressing GFP-tagged Ulp1^(C580S). Representative images indicating the localization of GFP-tagged Ulp1^(C580S)after G₂/M arrest are shown. The septin ring localization of Ulp1^(C580S)is indicated where present (arrowheads). Note that Ulp1^(C580S)failed to localize to the septin ring in SUMO-conjugating and ligating enzyme mutants (ubc9-1 and siz1Δ siz2Δ, respectively). (B) Septin ring localization of Smt3-GFP is absent in ubc9-1 and siz1Δ siz2Δ strains. Localization of Smt3-GFP was visualized in G₂/M-arrested WT, ubc9-1 and siz1Δ siz2Δ strains (YOK 1857, YOK 2144 and YOK 2143) using fluorescence microscopy. Arrowheads indicate the position of the septin ring.