Figure 4.

Fracture calluses from 5-LO^KO mice have enhanced mechanical properties. Fractured and contralateral femurs after 28 days (blue) and 84 days (green) of healing from control C57BL/6 mice (n = 11 at 28 and 84 days) and 5-LO^KO mice (n = 11 and 8 at 28 and 84 days, respectively) were mechanically tested to failure in torsion. Peak torque (panel A), maximum rigidity (panel B), maximum shear stress (panel C), and shear modulus (panel D) were calculated from the torque to angular displacement curves and callus dimensions. Fractured femur values were normalized to values from the contralateral femur of that mouse as a percentage. The limits of each rectangle represent the 25th and 75th percentile of the normalized values. The median value is indicated in each rectangle. The 5th and 95th percentile values are shown below and above each rectangle for those with group sizes of 11. Normalized values were compared between genotypes at each time point and p-values are indicated in each graph above the x-axis.