FIG. 3.

Combination CT plus CpG-ODN adjuvant enhances IgG2a production. Female BALB/c mice were immunized with MOMP mixed with either CT alone or both CT and CpG-ODN by direct application to skin as described in Materials and Methods. MOMP-specific IgG2a (upper panel) and IgG1 (lower panel) in serum (1:200 dilution) were determined by ELISA. Results are representative of three separate experiments. The y axis shows optical density at 450 nm. Error bars, standard deviations.