TABLE 2.

McCoy cells containing inclusions^a

Lavage fluid sample	Expt no.	% McCoy cells containing inclusions (mean ± SD)		P
		Nonimmune	Immune
Vaginal	1	57.6 ± 3.7	31.7 ± 3.6	<0.0002
	2	67.6 ± 6.7	24.4 ± 11.1	<0.01
Uterine	1	78.9 ± 1.2	39.3 ± 1.3	<0.0001
	2	75.3 ± 0.5	33.9 ± 1.6	<0.0001

^aC. muridarum was incubated for 1 h with vaginal and uterine lavage fluids from immunized or nonimmunized mice prior to plating onto McCoy cell monolayers. After 24 to 40 h of incubation, McCoy cell monolayers were fixed and stained to reveal chlamydial IBs. Results represent McCoy cells containing chlamydial inclusions. The dose of Chlamydia used resulted in infection of approximately 70% of the McCoy cells in the medium-only control cultures. All samples were tested in triplicate, and the results are representative of four separate experiments.