FIG. 1.

IFN-γ production by splenic cells from immunized and/or infected mice. Mice were immunized with CpG and H. pylori lysate or injected with PBS and challenged with H. pylori or PBS. At 3 months after the challenge, the mice were killed and the spleens were removed. Single-cell suspensions from the spleens of individual mice were restimulated in vitro with or without H. pylori lysate (10 μg/ml). Supernatants were harvested after 48 h and tested for IFN-γ and IL-4 by ELISA.