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. 2005 Nov 1;93(11):1257–1266. doi: 10.1038/sj.bjc.6602847

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Copyright © 2005 Cancer Research UK

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Construction scheme for the HuHMFG-1 Fab. Plasmids pAS1 and pAS2 containing the light chain and heavy chain of the HuHMFG-1 immunoglobulin were used as templates to PCR amplify the fragments for Fab construction. These were cloned into pET20b+ individually and NcoI/SalI and NcoI/NotI subgenes respectively. Then, the whole expression cassette for the light chain was amplified and cloned into the Bpu1102I site of the pET vector to form the pETHuHMFG1 expression vector.