Figure 3.

ARRDC3 is upregulated by fasting in humans and mice. (A) ARRDC3 expression in human subcutaneous adipose tissue biopsies from a microarray analysis. A randomized cross-over design was used to obtain paired fed and fasted data for each donor (P < 0.001; n = 10). (B) The data in panel A were combined with results of microarray analysis from a second group of 10 donors who underwent fasting on both occasions (combined P < 0.001; n = 20 fasted and 10 fed). (C) Confirmation of the effect of feeding on Arrdc3 expression by real-time PCR (***P < 0.001; n = 10/group). (D) Arrdc3 expression in multiple mouse tissues in response to a 14-hour fast (relative to mice fed ad libitum) (*P < 0.05; n = 3–4/group). (E) Northern analysis of tissues from fed and 14-hour fasting mice. Muscle, heart, kidney, brain, and spleen represent discontiguous lanes from a single blot. Error bars represent mean ± SEM. See also Figure S3.