Figure 5.

Arrdc3-deficient mice are resistant to the metabolic complications of obesity. (A) Fasting blood glucose levels of littermate wild-type (+/+), heterozygous (+/−) and homozygous (−/−) Arrdc3 knockout mice at 5–7 months of age. Male n = 6, 11, 3; female n = 7, 9, 6 for +/+, +/−, and −/− genotypes, respectively. (B) The same mice were then injected with low-dose (0.25 mU/g body mass) insulin into the peritoneum. Response of blood glucose levels to insulin is expressed relative to the baseline levels shown in panel A. (C) Glucose tolerance testing of male and female littermate mice at 5-6 months of age. Blood glucose levels were measured after an intraperitoneal injection of 2 g glucose/kg body mass. Male n = 6, 10, 3; female n = 5, 11, 7. (D) Insulin levels in fed mice at 3 months of age. Male n = 8, 10, 3; female n = 5, 13, 7. (E) Histological analysis of a 9-month-old male wild-type mouse and an Arrdc3 −/− littermate. Images are representative of those observed in 3 mice of each genotype. Scale bars, 10 μm. Error bars represent mean ± SEM; *P < 0.05; **P < 0.01 vs. wild-type.