FIG. 1.

Leptospiral proteins expressed by E. coli SOLR that contain plasmids rescued from phage clones. Mid-log-phase cultures were subjected to SDS-PAGE and immunoblotted with pools of sera from mares that had aborted (A) and from horses immunized with cultured leptospiras (B). Clones A1, B5, D7, and F5 produced an 80-kDa that was antigen reactive only with sera from mares that had aborted. A2 was an E. coli clone that did not express the antigen. Clone E8 expressed a leptospira protein that was reactive with both pools of sera. Proteins from each clone were loaded in duplicate. The lines represent alignment of the inserts from clones A1, B5, D7, and F5 and the 4.1-kb nucleotide sequence submitted to GenBank. lk73.5 and sph-like 2 are shown on the lower line. The positions of inserts of clones 2 and 8 were not determined.