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. 2004 Feb;72(2):742–749. doi: 10.1128/IAI.72.2.742-749.2004

FIG. 6.

FIG. 6.

Southern blot analysis of genomic DNAs from selected strains of Leptospira spp. DNA of B. burgdorferi, the genomic DNA of which has no sequence homologous to lk73.5, was used as a negative control. Genomic DNAs were digested with HindIII and probed with a biotin-labeled fragment of the 5′ terminus of lk73.5, which has less than 40% overall homology with other leptospiral sphingomyelinase genes. Lanes: BRA, L. interrogans serovar Bratislava; CAN, L. interrogans serovar Canicola; GRI, L. interrogans serovar Grippotyphosa; HAR, L. interrogans serovar Hardjo; COP, L. interrogans serovar Copenhageni; KEN, L. interrogans serovar Pomona type kennewicki; POM, L. interrogans serovar Pomona; BOR, L. borgpetersenii; INA, L. inadai; KIR, L. kirschneri; NOG, L. noguchi; WEI, L. weilii; BIF, L. biflexa; BUR, B. burgdorferi. The schematic on the top shows the positions of the single HindIII site and of the sequence used as a probe.