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MALDI-TOF mass spectrum of a trypsin digest of band 5 protein. Protein plugs from the gel were treated with trypsin by using an automated digester. The extracted peptides were desalted, mixed with an equal volume of saturated α-cyano-hydroxy-cinnamic acid in 30% acetonitrile-0.2% trifluoroacetic acid, and analyzed by MALDI-TOF mass spectrometry. The peak with an m/z of 2252.1667 is clearly visible. Relative intensities are given as percentages.
