FIG. 1.

Leishmania parasites induce DC migration and loss in vivo. The results of phosphatase staining of cryosections of spleens from control (PBS-injected) and mitogenic anti-CD3 (50 μg)- or parasite (10⁸)-injected (i.v.) C57BL/6 mice are shown. Times of injection are indicated. Sections were incubated with biotinylated antibodies against CD11c. The percentages of CD11C-positive cells present in spleen cells from mice of the same experiment were determined by cytofluorometry and are given in the upper right corner of each picture. These results are representative of three independent experiments. Bar, 150 μm.