FIG. 3.
Leishmania parasite-mediated DC maturation requires live parasites. C57BL/6 mice were injected (i.v.) with a control solution (PBS), 108 syngeneic spleen cells, L. donovani culture supernatant, 108 heat-killed L. donovani parasites, or 108 viable L. donovani parasites (as indicated in the figure). At 9 h after treatment, mice were sacrificed and spleens were collected. Pooled low-density spleen cells from three individuals in each group were double stained with PE-labeled anti-CD11c and with biotinylated anti-CD86 followed by streptavidin-APC treatment. The data represent the fluorescence of cells gated for high levels of CD11c expression. These results are representative of two independent experiments.