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. 2004 Feb;72(2):844–852. doi: 10.1128/IAI.72.2.844-852.2004

FIG. 2.

FIG. 2.

Immunoblots of C. Burnetii antigens with infection-derived mouse sera. (A) Immunoblot of phase I C. burnetii antigen with sera from mice infected with various doses of C. burnetii. Lane 1, uninfected mouse sera. Lanes 2 to 4, weeks 2, 3, and 5, respectively, postinfection with 106 organisms. Lanes 5 to 7, weeks 2, 3, and 5, respectively, postinfection with 108 organisms. Lanes 8 to 10, weeks 2, 3, and 5, respectively, postinfection with 109 organisms. (B and C) Immunoblots of proteinase K-digested and undigested phase I and phase II WCAs with mouse sera from weeks 2 and 5, respectively, postinfection with 108 organisms. WC, whole cell; WCL, whole-cell lysates. (D) Immunoblot showing IgM activity against proteinase K-digested and undigested phase I antigens in 1:25-diluted mouse sera from week 5 postinfection. PK-I, proteinase K-treated phase I antigen.