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. 2004 Feb;72(2):844–852. doi: 10.1128/IAI.72.2.844-852.2004

FIG. 4.

FIG. 4.

Comparison of reactivities of recombinant C. burnetii antigens expressed by different clones in E. coli XLOLR with acute- and convalescent-phase antisera from mice experimentally infected with various doses of C. burnetii. (A) Immunoblot of recombinant proteins with sera from 2 weeks postinfection (acute phase) with 105 organisms. (B) Immunoblot of recombinant proteins with sera from 5 weeks postinfection (convalescent phase) with 109 organisms. The samples in panels A and B are the same. Lane 1, Nine Mile phase I lysates; lanes 2 to 18, lysates of E. coli XLOLR containing different recombinant plasmids encoding the following ORFs: lane 2, 01453; lane 3, 01019; lanes 4 and 5, 00518; lane 6, 01633; lanes 7 and 9, 02027; lane 8, 02428; lanes 10 and 11, 01632; lane 12, 02703; lane 13, 02705; lane 14, 00701; lane 15, 01174; lanes 16 to 18, 01173; lane 19, negative control, lysates of E. coli XLOXR containing pBK-CMV plasmid.