Table 4.
qPCR confirmation of age-related induction of MHC II pathway component genes
| Gene | All Aged vs. Adult (ratio, p-value t-test) | Pearson Correlation to Morris water maze | Aged Intact vs. Adult (ratio, p-value SNK) | Aged Impaired vs. Adult(ratio, p-value SNK) |
Aged Impaired vs. Aged Intact(ratio, p-value SNK) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Syn | CA1 | CA3 | DG | Syn | CA1 | CA3 | DG | Syn | CA1 | CA3 | DG | Syn | CA1 | CA3 | DG | Syn | CA1 | CA3 | DG | |
| MHC II Components | ||||||||||||||||||||
| Cd74 | 6.58### | 5.61### | 3.61### | 2.43### | - | - | - | - | 6.79*** | 5.66*** | 3.88*** | 2.43*** | 6.35*** | 5.58*** | 3.40*** | 2.49*** | - | - | - | - |
| Ctse | 1.69## | 1.45## | 1.75### | 1.48### | - | - | - | - | 1.81* | - | 1.73*** | 1.48* | - | - | 1.77*** | 1.54** | - | - | - | - |
| Hla-dmb | 1.64### | 1.37### | 1.37### | 1.26## | - | - | - | - | 1.57** | 1.33** | 1.39*** | - | 1.72*** | 1.40*** | 1.35*** | 1.33** | - | - | - | 1.16* |
| Hla-dra | 6.30### | 4.04### | 3.02### | - | - | - | - | - | 6.76*** | 4.18*** | 3.25*** | - | 5.75** | 3.95*** | 2.85*** | - | - | - | - | - |
| Hla-drb1 | 10.50### | 7.13### | 4.39### | 2.91# | - | - | - | - | 10.70*** | 7.12*** | 4.77*** | - | 10.26*** | 7.13*** | 4.12*** | 3.42** | - | - | - | - |
| Antigen Recognition | ||||||||||||||||||||
| C1s | 1.84# | 1.21# | 1.56### | - | - | - | - | - | - | - | 1.59** | - | - | - | 1.53*** | - | - | - | - | - |
| C3 | 3.47### | 2.00### | 1.96### | 1.69### | - | - | - | - | 3.40** | 2.07*** | 2.22*** | 1.52*** | 3.55** | 1.94*** | 1.78*** | 1.81*** | - | - | - | 1.19* |
| C4a | 4.18### | 1.77### | 1.51### | - | - | - | - | - | 3.83** | 1.67*** | 1.58*** | - | 4.59** | 1.85*** | 1.47*** | - | - | - | - | - |
| Serping1 | 2.71### | 2.03### | 2.27### | 1.54### | - | - | - | - | 2.79*** | 2.18*** | 2.52*** | 1.49** | 2.63*** | 1.92*** | 2.09*** | 1.58*** | - | - | - | - |
| Tlr2 | 1.64## | - | 1.54## | - | - | - | - | - | 1.55* | - | 1.66* | - | 1.73* | - | 1.46* | - | - | - | - | - |
| Tlr4 | 1.51## | 1.35# | 1.96### | - | - | - | - | - | 1.41* | - | 2.00*** | - | 1.60* | - | 1.93*** | - | - | - | - | - |
| Tlr7 | 1.69## | 1.34## | 1.73### | - | - | - | - | - | 1.65* | 1.34* | 1.74*** | - | 1.73* | 1.35* | 1.72*** | - | - | - | - | - |
| Immune Cell Activating | ||||||||||||||||||||
| Ccr5 | 1.96# | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| Erbb3 | 1.28### | 1.34### | 1.20# | 1.40# | - | - | - | - | 1.26** | 1.43*** | - | - | 1.30** | 1.28** | - | 1.47** | - | - | - | - |
| Fcgr2a | 1.45# | 1.19# | 1.30### | - | - | - | - | - | - | 1.30** | - | - | - | 1.30** | - | - | - | - | - | |
| Fcgr2b | 1.96## | - | 1.61### | - | - | - | - | - | 2.08* | - | 1.63*** | - | 1.82* | - | 1.60*** | - | - | - | - | - |
| Inflammation Response | ||||||||||||||||||||
| Cp | 1.67# | - | 1.56### | - | - | - | - | - | 1.92* | - | 1.64*** | - | - | - | 1.50*** | - | - | - | - | - |
| Hsbp1 | 2.87### | 1.68### | 2.09### | - | - | - | - | - | 3.13** | 1.65** | 2.25*** | - | 2.58*** | 1.70** | 1.97*** | - | - | - | - | - |
| Icam1 | 1.46# | 1.55### | 1.43### | - | - | - | - | - | - | 1.68*** | 1.64*** | - | - | 1.46** | 1.31** | - | - | - | - | - |
| Lgals3 | 3.42## | 2.64### | 1.98### | 1.84## | - | - | - | - | 3.68*** | 2.62*** | 2.18*** | 1.63* | 3.03*** | 2.66*** | 1.84*** | 2.00** | - | - | - | - |
| S100a6 | 1.52# | 2.03### | 2.21### | - | - | - | - | - | - | 2.00*** | 2.48*** | - | - | 2.06*** | 2.02*** | - | - | - | - | - |
qPCR confirmation of transcriptomic findings was performed in hippocampal synaptosomes and expanded to include hippocampal subregion dissections. Age-based comparisons (aged intact and aged impaired versus adult) were statistically analyzed by two-tailed t-test with BHMTC (#p < 0.05; ##p < 0.01; ###p < 0.001). Three-group data (adult, aged intact, aged impaired) were statistically analyzed by one-way ANOVA with BHMTC, and pairwise comparisons were evaluated by SNK post hoc testing to identify potential regulation of gene expression with cognitive decline (*p < 0.05; **p < 0.01; ***p < 0.001). Correlation of gene expression with Morris water maze performance was assessed by Pearson product moment analysis with BHMTC, which demonstrated no significant association of MHC II pathway gene expression and cognitive impairment. Data are presented as ratios of group means. Individual group gene expression data is presented in Table 5.