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. 2011 Jun 27;193(7):1213–1228. doi: 10.1083/jcb.201103076

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© 2011 Bizzari and Marston

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Kinetochores are monooriented during meiosis I in cdc55mn cells. (A–D) Wild-type (AM5900; A and C) and cdc55mn (AM5771; B and D) cells carrying MAM1-9MYC, NDC10-6HA, GAL-NDT80, and pGPD1-GAL4(848).ER were released from a pachytene block, and the localization of Mam1-9Myc was determined using Ndc10-6HA as a marker for kinetochores. (C and D) The percentages of binucleate and tetranucleate cells determined at the indicated time points are shown for a representative experiment. (E–H) Strains carrying heterozygous CEN5-GFP dots and otherwise cdc14-1 (AM6910), cdc14-1 cdc55mn (AM6934), cdc14-1 mam1Δ (AM7355), and cdc14-1 mam1Δ cdc55mn (AM7328) were analyzed as described in Fig. 3 (C–F).