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. 2011 Jun 27;193(7):1213–1228. doi: 10.1083/jcb.201103076

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© 2011 Bizzari and Marston

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Equational segregation occurs on a newly assembled spindle in cdc14-1 mutants and is elevated by Cdc55 depletion. (A and B) Strains carrying heterozygous CEN5-GFP, GAL-NDT80, pGPD1-GAL4(848).ER, and either cdc14-1 (AM8044; A) or cdc14-1 cdc55mn (AM8045; B) were released from a pachytene block, and the percentages of cells with the indicated spindle morphology (top graph) and pattern of CEN5-GFP localization in the binucleate cells (bottom graph) were determined. (C–F) cdc14-1 (AM7866) and cdc14-1 cdc55mn (AM7867) cells carrying URA3-tdTomato (red) and GFP-TUB1 (green) were imaged at 10-min intervals for a total of 9 h and 46 min and 9 h and 56 min, respectively. (C) Example of a cdc14-1 cell that completes a single meiotic division. (D) Example of a cdc14-1 cdc55mn cell that completes two divisions on the same axis. Times at the top are given in minutes. Bars, 1 µm. (E) Behavior of cdc14-1 and cdc14-1 cdc55mn cells that were initially in prophase I and which performed at least one division during the course of filming. red, reductional; equ, equational. (F) The frequency of cdc14-1 and cdc14-1 cdc55mn cells that performed the second division within the indicated time period. Also see Videos 4 and 5. (G and H) Strains carrying heterozygous CEN5-GFP dots GAL-NDT80, pGPD1-GAL4(848).ER, and either spo11Δ cdc14-1 (AM8046; G) or spo11Δ cdc14-1 cdc55mn (AM8047; H) were treated as described in A and B.