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. 2011 Nov 2;2011:543512. doi: 10.1155/2011/543512

Figure 4.

Figure 4

Changes in the mRNA levels of NF-68 and 200 in primary cultures of CGCs exposed to 20 μM domoic acid alone or in combination with antagonists for the AMPA-R (NBQX, 20 μM), NMDA-R (APV, 100 μM) or the neurotransmitter GABA (10 μM) at 1 DIV (immature cultures) or at 7 DIV (mature cultures) for 3 or 10 days. Note that NBQX but not APV prevent the domoic acid-induced downregulation of (a) NF-68 in immature cultures, (b) NF-68 in mature cultures, (c) NF-200 mRNA expression in immature cultures, and of (d) NF-200 in mature cultures. Gene expression levels were normalized to the standard calibrator, the housekeeping gene 18S rRNA and the mRNA expression at 1 DIV. Data represent mean ± S.E.M. of three independent experiments performed in duplicates.   *P < 0.05  **P < 0.01  ***P < 0.001 comparing treated to control (untreated) cultures.